Correction: A Cell Culture Adapted HCV JFH1 Variant That Increases Viral Titers and Permits the Production of High Titer Infectious Chimeric Reporter Viruses
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منابع مشابه
A Cell Culture Adapted HCV JFH1 Variant That Increases Viral Titers and Permits the Production of High Titer Infectious Chimeric Reporter Viruses
The unique properties of the hepatitis C virus (HCV) JFH1 isolate have made it possible to produce and study HCV in an infectious cell culture system. However, relatively low virus titers restrict some of the uses of this system and preparing infectious chimeric reporter viruses have been difficult. In this study, we report cell culture-adapted mutations in wild-type JFH1 yielding higher titers...
متن کاملA milestone for hepatitis C virus research: a virus generated in cell culture is fully viable in vivo.
C hronic hepatitis C virus (HCV) infection is a leading cause of end-stage liver disease in the United States, and the Centers for Disease Control has predicted that 10,000 Americans die annually from this disease. Since the discovery of HCV in 1989, basic research on this important pathogen has been difficult because of the lack of a robust cell-culture system. Thus, the recent development of ...
متن کاملPermissivity of Primary Human Hepatocytes and Different Hepatoma Cell Lines to Cell Culture Adapted Hepatitis C Virus
Significant progress has been made in Hepatitis C virus (HCV) culture since the JFH1 strain cloning. However, developing efficient and physiologically relevant culture systems for all viral genotypes remains an important goal. In this work, we aimed at producing a high titer JFH1 derived virus to test different hepatic cells' permissivity. To this end, we performed successive infections and obt...
متن کاملCharacterization of the early steps of hepatitis C virus infection by using luciferase reporter viruses.
The lack of an efficient system to produce hepatitis C virus (HCV) particles has impeded the analysis of the HCV life cycle. Recently, we along with others demonstrated that transfection of Huh7 hepatoma cells with a novel HCV isolate (JFH1) yields infectious viruses. To facilitate studies of HCV replication, we generated JFH1-based bicistronic luciferase reporter virus genomes. We found that R...
متن کاملProduction of Infectious Hepatitis C Virus of Various Genotypes in Cell Culture Running Title; Infectious HCV Production in Cell Culture
A unique hepatitis C virus (HCV) strain JFH-1 has been shown to replicate efficiently in cell culture with production of infectious HCV. We previously developed a DNA expression system containing HCV cDNA flanked by two self-cleaving ribozymes to generate HCV particles in cell culture. In this study, we produced HCV particles of various genotypes including 1a (H77), 1b (CG1b) and 2a (J6 and JFH...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/annotation/2e81c9a3-4700-4869-aee9-f079092c77cd